Antagonistic Properties of Methanol Extract of Elaeocarpus sphaericus Leaves against in vitro Growth, Germ Tube Formation, Adhesion and Biofilm of Candida Species
By: Gupta, P
.
Contributor(s): Rastogi, A | Joshi, S.
Publisher: Mumbai Indian Journal of Pharmaceutical Science 2018Edition: Vol. 80(05), September-October.Description: 949-954.Subject(s): PHARMACEUTICS | Candida glabrata | Biofilm | XTT assay | Broth microdilutionOnline resources: Click here
In:
Indian journal of pharmaceutical sciencesSummary: The extracts of Elaeocarpus sphaericus (Roxb.) have shown antimicrobial activities against many pathogenic microbes including fungi. However, the effects of E. sphaericus have not been investigatd against key virulence attributes of Candida. In the present study, methanol extract of E. sphaericus leaves was analyzed against planktonic cells, hyphal growth, adhesion and biofilm formation of two most important fungal pathogens, Candida albicans and Candida glabrata. Methanol extract of E. sphaericus leaves was prepared by pressurized liquid extraction method. Inhibitory concentration of methanol extract of E. sphaericus leaves for both pathogens was determined by broth microdilution assay. Germ tube formation assay was performed to study the effect of the extract on hyphal transition. Effect of methanol extract of E. sphaericus leaves on adhesion and biofilm formation was investigated in microtiter plate using 2,3-bis(2-methoxy-4-nitro-sulfophenyl)-2H-tetrazolium-5-carboxanilide reduction assay. IC50 values of the extract for C. albicans and C. glabrata were found between 0.625-1.25 mg/ml. Serum-induced germ tube development in C. albicans was inhibited in presence of 5 mg/ml methanol extract . The extract remarkably inhibited in vitro adhesion and biofilm formation by C. albicans and C. glabrata in microtiter plates. The results of this study showed anticandida potential of methanol extract of E. sphaericus leaves.
| Item type | Current location | Call number | Status | Date due | Barcode | Item holds |
|---|---|---|---|---|---|---|
Articles Abstract Database
|
School of Pharmacy Archieval Section | Not for loan | 2018341 |
The extracts of Elaeocarpus sphaericus (Roxb.) have shown antimicrobial activities against many pathogenic microbes including fungi. However, the effects of E. sphaericus have not been investigatd against key virulence attributes of Candida. In the present study, methanol extract of E. sphaericus leaves was analyzed against planktonic cells, hyphal growth, adhesion and biofilm formation of two most important fungal pathogens, Candida albicans and Candida glabrata. Methanol extract of E. sphaericus leaves was prepared by pressurized liquid extraction method. Inhibitory concentration of methanol extract of E. sphaericus leaves for both pathogens was determined by broth microdilution assay. Germ tube formation assay was performed to study the effect of the extract on hyphal transition. Effect of methanol extract of E. sphaericus leaves on adhesion and biofilm formation was investigated in microtiter plate using 2,3-bis(2-methoxy-4-nitro-sulfophenyl)-2H-tetrazolium-5-carboxanilide reduction assay. IC50 values of the extract for C. albicans and C. glabrata were found between 0.625-1.25 mg/ml. Serum-induced germ tube development in C. albicans was inhibited in presence of 5 mg/ml methanol extract . The extract remarkably inhibited in vitro adhesion and biofilm formation by C. albicans and C. glabrata in microtiter plates. The results of this study showed anticandida potential of methanol extract of E. sphaericus leaves.
Click on an image to view it in the image viewer
There are no comments for this item.